Order: Cetacea
Family: Delphinidae

1) General Zoological Data

There is a single species of Orcinus that populates all oceans; the former subdivisions have been abandoned. This is the largest member of the Delphinidae. In addition to their wide distribution, numerous aquariums exhibit killer whales and many breed this species. This social animal occurs in schools of up to 250 animals and it is also easily ‘trained' to perform. Females weigh up to 5,500 kg, males up to 9,000 kg, according to Nowak (1999). Weigl (2006) described maximum longevity as at least 34 years, but Nowak indicated longevity to 50-60 years. Gotch (1979) derived the name from the Latin: whale=orca .

Thewissen ed. (1998) suggested that the earliest whales specimens recovered are from 50 MYA and he traced the development of these animals from the time of their appearance in a remarkable book. Recent studies by Nikaido et al. (1999) have shown the close genetic relationship between hippopotamus and cetacea. A wide variety of web sites and internet references exist for this species.

	Killer whale with calf.

2) General Gestational Data

The single newborns weigh around 180 kg and are born after a gestation period of 517 days (Nowak, 1999). Robeck et al. (2004) researched the reproductive and endocrine physiology of killer whales with the intent of performing artificial insemination. They did this twice, and they also described the semen quality.

3) Implantation

The cetacean placenta is an epitheliochorial, diffuse organ that implants all over both uterine horns. It fills the entire uterus, with the umbilical cord inserting near the endocervical os. The time of implantation following fertilization, however, remains unknown. The main body of the fetus occupies one horn, while the tail occasionally extends into the other horn. The intrauterine position of the cetacean fetus has been illustrated by Slijper (1956, 1966). The position of the fetus is generally such that the tail delivers first. Wislocki & Enders (1941) described the placenta of bottlenose dolphins (see the chapter on dolphins).

4) General Characterization of the Placenta

I have had three specimens of killer whale placentas for examination (Benirschke & Cornell, 1987), the only reports other than that by Turner (1872) who had an entire uterus with fetus for study. In contrast to his remarks about findings ‘naked' ends of the chorionic sac, ours had villi covering these tips. Turner's fetus was located in the left uterine horn, while the corpus luteum was found in the right ovary. The placenta extended into both horn; being smaller in the horn that did not contain the fetus. In general, however, odontocetes have their ovulation in the left ovary (Benirschke et al., 1980).

	Layout of killer whale placenta with central cord insertion and allantoic sac.
	Diagram of this placenta with its measurements (from Benirschke & Cornell, 1987).
	The ‘tip' of the placenta (top left) is covered with villi; a distinct ridge extended over the chorionic plate.
	Chorion with large vessels and villi extending peripherally.
	Chorion with villi and small chorionic vessels.
	Two villi arising from the chorion. They are covered by a single-layered trophoblast layer.

5) Details of fetal/maternal barrier

The entire villous periphery is covered with a single layer of cuboidal trophoblast; there are no denuded areas. The endometrium is covered with epithelium that is not eroded. Thus, the barrier is complete epitheliochorial. The trophoblast is underlain by numerous fetal capillaries, has fine PAS positive pigment granules, and has frequent vacuoles.

	Details of villi with single layer of trophoblast and large number of fetal capillaries.

6) Umbilical cord

The umbilical cord was short, ~80 cm long and had no turns; it was 4 cm in diameter. The cord inserted near the endocervical canal; it apparently ruptured spontaneously during delivery near its insertion on the fetus. The normal umbilical cord contains two large arteries and two large veins, without major anastomoses between any of the vessels. The umbilical vessels branch at the placental insertion dichotomously towards the periphery of the placenta. In addition to the major umbilical vessels, there is a profusion of small and medium-sized blood vessels in the umbilical cord, as many as up ~hundreds in cross-sections. Many have no musculature and are then scattered irregularly through the cord. They have no particular predilection to locate around the allantoic duct. Injection with radio-opaque dyes of the large veins and arteries (undertaken in a bottlenose dolphin) discloses that the small vessels originate from the large ones and also, that they communicate among the large vessels. The function of these small vessels is unknown. The central portion of the umbilical cord carries an irregularly shaped allantoic duct with sparse, and usually very thin epithelium. The Wharton's jelly is sparse and unusually firm. On the umbilical surface, foci of squamous metaplasia (“caruncles”) are common. They are often remarkably pigmented, both black and white. These ‘plaques' of squamous metaplasia extended some distance onto the amnion but were there more widely disseminated.

	Cross section of the umbilical cord with 2 arteries, 2 veins and a central allantoic duct (A.D.).
	Pigmented and unpigmented foci of squamous metaplasia on the surface of the umbilical cord.
	This is the edge of the cord's allantoic duct with low (detached) epithelium.
	Plaque on the cord surface with extensive squamous metaplasia. Note the pigmented basal epithelium and numerous basal capillaries.

7) Uteroplacental circulation

There are no specific publications dealing with either the uterine circulation or its physiology, but the arrangement of the pelvic vasculature was discussed by Rommel and colleagues (1993). They described that the uterus is surrounded by a complex countercurrent system of blood vessels, which, they suggested, is being used for cooling purposes. There is also an extensive consideration of various other circulatory aspects in their description.

8) Extraplacental membranes

There are no free membranes, but amnion and allantoic sacs are contained within the chorionic sac. The allantoic duct within the umbilical cord is lined by columnar or cuboidal epithelium and is also accompanied by bundles of smooth muscle. This “jelly roll” shown next was prepared from the interface of the membranes; it has the amnion inside which has a very simple, flat epithelium. There were no vessels in the allantoic membrane. The allantois was cannulated through the allantoic duct in the umbilical cord, thus confirming the arrangement of the diagram shown earlier. Several very small pieces of presumed ‘hippomanes', crumbling material of darker color, were present in the allantoic sac of one specimen.

	Amnionic and allantoic membranes without vessels and composed of connective tissue and thin epithelium.
	Heavily pigmented allantoic debris (‘hippomanes') of one specimen.

9) Trophoblast external to barrier

There is no invasion of the uterus by trophoblast.

10) Endometrium

No decidua is present in cetacea. There is an “enormous development of superficial capillaries as well as large numbers of racemose glands” in the endometrium (Slijper, 1966). The microscopic anatomy of the female genital tract, including the endometrium, has been described for marine mammals in general by Simpson and Gardner (1972). It includes a description of the arterial supply, as well as of the cervix and the ovaries.

11) Various features

The myoglobin of killer whales was studied by Castillo et al. (1977); it resembled that of the sperm whale more than porpoises. Electronmicroscopic studies on semen were performed by Miller et al. (2002).

12) Endocrinology

I am aware of only few endocrine studies in killer whales; however, Hobson & Wide (1986) extracted chorionic gonadotropin from a bottlenose dolphin placenta. Whether delphinidae produce functional placental gonadotropins is currently being examined in more details by Japanese scientists. Robeck et al. (2004) measured estrogens, progestins and LH in killer whales in anticipation of performing artificial insemination. Robeck & Monfort (2006) characterized the male killer whale endocrine physiology with testosterone measurements. More recently, Kusuda et al. (2011) have determined the length of the ovarian cycle in a single killer whale by observing rectal temperature and progesterone levels. By these observations the length of the cycle is approximately 44 days.

13) Genetics

A number of chromosome studies have been performed on killer whales; they are summarized by Hsu & Benirschke (1973) from which the following figure comes. Killer whales have 44 chromosomes; an excellent banding study was done on six killer whales that showed considerable polymorphism of C-bands; this is shown in the following picture (Arnason et al., 1980). Hybrids have not been reported to the best of my knowledge. It is interesting, however, that now a web-based DNA-identification system has been developed (Ross et al., 2003) for ready identification of individual specimens. It is universally available. There have also been studies to ascertain how killer whales in pods avoid inbreeding. On the other hand, Hoelzel et al. (1998; 2002) studied the genetic variation worldwide of killer whales and found relatively little genetic variety.

	Karyotypes of male and female specimens (from Hsu & Benirschke, 1973).
	Karyotype (C-banded) of female killer whale (from Arnason et al., 1980).

14) Immunology

I am not aware that any studies have been published other than the review of immunoglobulin concentrations by Taylor et al. (2002).

15) Pathological features

Ridgway (1979) has summarized major causes of death in killer whales from oceanaria and presents a wide variety of lesions. Various infections have been identified in delphinidae, including the killer whale (e.g. Robeck & Dalton, 2002). Brucella infection has been reported from bottlenose dolphins (Miller et al., 1999) and may also occur in killer whales, although direct confirmation from antibody studies is not confirmatory (Omata et al., 2006). Various investigators have identified environmental toxins/contaminants I odontocetes (Minh et al., 2000 a,b; Koopman et al., 2003. In reviewing material published in web sites of cetacea it appears that killer whales often have tapeworms, roundworms and flukes. Also, Hodgkin's disease has been reported (Yonezawa et al., 1989) and severe coronary arteriosclerosis was found in a stranded specimen. Murata et al. (2004) identified toxoplasma antibodies in some captive animals.

16) Physiologic data

There are extensive data collected in the book by Ridgway (1972) for many physiological characters.

17) Other resources

No specific other anatomic studies are reported.

18) Other remarks – What additional Information is needed?

Modern studies of an implanted placenta are desirable.

Acknowledgement

The animal photograph in this chapter comes from Dr. Sam Ridgway, San Diego .

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